The purpose of this research is to analysis of influence service quality toward patients satisfaction in Puskesmas Jogonalan, Klaten. The population of the research is all patients in Puskesmas Jogonalan. Sample was taken with purposive technic sampling for about 120 people. Research data was obtained through questionnaire. Analysis data was done using multiple linear regression after doing validity and reliability fest. The test result was done by showing direct evidence, reliability, guarantee, responsiveness, and empathy are together significantly influencing the patient satisfaction in Puskesmas Jogonalan, Klaten. The direct evidence gives positive and significant influence toward the patient satisfaction. Reliability gives positive and significant influence toward the patient satisfaction in Puskesmas Jogonalan, Klaten. The responsiveness gives positive and significant influence toward the patient satisfaction. Guarantee gives positive and significant influence toward the patient satisfaction. Empathy gives positive and significant influence toward the patient satisfaction in Puskesmas Jogonalan, Klaten.