Background: infectious diseases caused by Escherichia coli can be treated with natural remedies using tea extract and honey, because the tea extract contains catechins as anti-bacterial. While honey contains an acidic pH that can inhibit bacterial growth. Objective: Knowing the number of hepatic bacteria and the effective dosage of combination tea extract and honey on rats liver that has been in the infection of Escherichia coli. Research Method: The study design was laboratory experimental with the post test-only control group. This research was conducted in UGM Laboratory for 2 weeks in Februri-March 2018. The subjects of this study were white rats as many as 30 tails, divided into 5 groups (n = 6), K1 control group negative (not infected), group K2 positive control (infected with Escherichia coli without tea and honey extract), P3-P5 group (given tea and honey extract 50% -50%, 75% -25%, and 25% -75%). Result: The lowest average number of liver in the rats bacteria was infected Escherichia coli and given 50% tea extract solution - 50% honey by 2.63 x 103 CFU/ gram. The results of One Way Anova parametric test is p <0.05. This means that giving a combination of tea extract and honey affect the number of rats liver germs in infections Escherichia coli. Conclusion: The effective dose of combination of tea extract and honey that can influence the number of hepatic bacteria in rats in Escherichia coli infection is 50% - 50%.