Back ground: Based on data from the 2014 National Health Profile there are 74.9% of districts and cities that have carried out waste management in hospitals but have not achieved the 2014 Ministry of Health's Renstra target of 75%. The percentage of puskesmas that have managed the correct medical waste is only 55% in 2014 Research method: This research is a quantitative study with observational analytic design using a cross sectional design. The subjects of this study were health workers at the puskesmas. The number of samples is 50 respondents, the sampling technique uses totallity sampling. The instrument in this study is using a questionnaire and checklist. Data analysis using descriptive statistical test and chi square test Results: The results of this study indicate that based on the knowledge of health workers working Community Health Center, most of them were in the good category as many as 24 respondents (60.0%). most of them are included in the yes category as many as 29 respondents (72.5%). This study also shows that there is a relationship between knowledge and compliance with waste management of health workers working Health Center, with a significance value on the results of the study (p = 0.002 <0.05) Conclusion: there is a correlation between knowledge and compliance with waste management of health workers working Community Health Center, with a significance value on the results of the study (p = 0.002 <0.05)