Background: Estrogen has an important role in regulating the menstrual cycle and reproduction. Decreased estrogen hormone and secretions from the hormone progesterone can occur during menopause. The hormone estrogen can affect vascular permeability that has vasodilation. This change in permeability can cause bacterial invasion which causes irritation or infection in the oral cavity and can eventually cause ulcers. The role of fibroblasts on wound healing is responsible for preparing the production of protein structure products that will be used in the process of tissue reconstruction. To help overcome the problem regarding the deficiency of the hormone estrogen, namely by using phytoestrogen compounds in plants. Phytoestrogens can be classified as isoflavonoids and lignans. Isoflavones are a group of phytoestrogens which are similar to chemical structures similar to those of estrogen in mammals. Phytoestrogens have the same structure and function as endogenous estrogens. So it can be concluded that phytosterogen can reduce menopausal symptoms. Besides being able to reduce menopausal symptoms, the flavonoid content in phytoestrogens can inhibit cyclooxygenase or lipookigenase and inhibit leukocyte accumulation so that it can become antiinflammatory in the process of wound healing. Method: This type of research is Experimental Laboratory in experimental animals with Sprague Dawley rats totaling 20 individuals divided into 4 treatment groups and 1 control group observed in several times namely 1 day, 3 days, 5 days and 7 days then seen changes in cell number fibroblasts in each group with a light microscope with a magnification of 100x with staining of hematoxylin-eosin (HE). Results: In the four samples of each treatment group when tested the statistical data showed that on the seventh day the statistical data showed that the significance value was 0.748 so that there were no significant differences between the five groups.Conclusion: There is no effect of soybean flour extract on the number of fibroblast cells in traumatic ulcers in female Sprague Dawley mice with estrogen deficiency.