Research about the effect of the Quality Management System ISO upon employee's performance through quality corporate culture can be seen from three dimensions. The three dimensions are ISO 9001 certification planning, corporate commitment and procedure implementation. With Puskesmas Mantrijeron Yogyakarta as the object and 50of all employees as the respondent in collecting the data, this research found the result. This study was followed by testing the extent to whichthe influence implementation of Quality Management System ISOto employee's performance through the quality culture. The analysis technique used is Partial Least Square (PLS). According to the employees, ISO 9001 certification planning, corporate commitment, and procedure implementation have been running well. It also gives the significant positive effects upon quality corporate culture. Furthermore, the certification planning and corporate commitmentdoes not significantly influence to employee's performance.