2013 batch Basic Dentistry Bloc’s OSCE passing percentage on teeth identifying station is 65,48%, while for 2015 batch is 59,4%. The effort that can be done to increase the interest of study is through interactive studying medium. Tooth Morphology Board Game is a studying medium introduced firstly to PSPDG FKIK UMY students. Evaluation is required to assess its function as newly introduced studying medium. The evaluation is using students’ responses or perception after using the studying medium. Goals: To know the relation between perception with the knowledge level of PSPDG FKIK UMY first year students in learning teeth morphology concept after using tooth morphology board game studying medium. Method: Cross sectional study is done to 30 subjects which were PSPDG FKIK UMY students after playing tooth morphology board game three times. Data collecting method uses purposive sampling, while data analysis uses lambda correlation test and chi-square. Result: From 30 research subjects, the result is 18 subjects (60%) are in favor that consists of 6 (20%) standard knowledge level students and 12 (40%) good knowledge level students. On the other side, 12 subjects (40%) are not in favor that consists of 1 (3,3%) low knowledge level student, 8 (26,7%) middle knowledge level students, and 3 (10%) high knowledge level students. Using lambda correlation test, the result is the correlation 0,296 categorized as low and the contribution is 8,76%. Chi-square test result gets the significant correlation. Conclusion: There is a correlation between perception and knowledge level of PSPDG FKIK UMY Year I students in learning morphology concept after using tooth morphology board game studying medium.