Wound is the loss of integrity of the skin epithelium. The wound is an injury that is often experienced by every human being. Wound healing process is divided in 3 phases: 1). Hemostasis and inflammation, 2). Proliferation and 3). Maturation and remodeling.Proliferation phase which happens at the 5th-21st day will increase after Fibroblasts wound happens. Black cumin (Nigella Sativa) has an active substance that can increase the number of fibroblast cells namely Saponin, Flavonoid and Thymoquinone. The effect of saponins on fibroblasts is to synthesize fibronectin which can accelerate wound healing. The type of this study was experimental laboratory invivo with post test only control group design design.Type of this research was experimental of invivo laboratory with the design of post-test only control group. This research used 36 mice which were divided being 3 treatment groups; those were positive control (aloclair), treatment control (jintanhitam gel), and negative control (CMC-Na). Gingiva mice were injured using a scalpel with a diameter of 5 mm. After that, gingival tissue was taken and made preparation. Observation with 20x40 magnification with 10x viewable field using a light microscope. The result of this study werw tested by Kruskal-Wallis with the result that there was a significant (p<0,05) difference to the increase of the number of fibroblast cells between 3 treatment groups based on the action. The Kruskal-Wallis test based on dy showed no significant (p>0,05) result from each treatment group. This study showed that Nigella sativa gel can increase the number of fibroblast cells to the wound healing process in rat gingiva.