Background: The incidence of tuberculosis (TBC) in healthworkers in developing countries reaches approximately 69 to 5,780 per 100,000 people each year depending on the location of work and jobs. Meanwhile, Influenza suffered by nearly 50% of health workers every year. One of the efforts to prevent and minimize the incidence of tuberculosis, common cold, and infections transmitted through the air, droplet or sprinkling of body fluids are use of mask and apply the cough ethic and respiratory hygiene. Knowledge and attitudes about use of mask and cough ethic and respiratory hygiene can be obtained by education and training program. They are associated with decreasing the spread of infection and the incidence of respiratory tract infections in healthworkers. Method: This study is an analytic quantitative study that uses Quasi Experimental design with non randomized control group pretest posttest design approach. The samples in this study are 32 peoples consisting of 16 respondents in the treatment group and 16 respondents in the control group. The assessments are using multiple choice knowledge questionnaire and attitude with likert scale and the result is analyzed by using Wilxocon and Mann-Whitney test. Results: The Wilcoxon analysis test showed that there is a significant difference between pre-test and post-test knowledge (p = 0.007, <0.05) and attitude (p = 0.003, <0.05) of respondents in the treatment group and the control group. Based on the result of Mann-Whitney test on the change of knowledge and attitude score, the value of significance is p = 0.032 (<0.05) and p = 0.015 (<0.05) which means that the respondents who have been given treatment in the form of education have increased the score of knowledge as much 81.2% and attitude of 87.5% in the treatment group. Conclusion: This research can be concluded that education about use of mask influence to knowledge and attitude of paramedic at Puskesmas Gamping I and Sewon II.