There are many art culture in Daerah Istimewa Yogyakarta such as food, tourist destination, traditional architecture, traditional costume, dances, traditional weapon, ethnic, traditional language and song. But not all of art and culture may be protected by the law, the aim of law protection is to make the art and culture a hallmark of Yogyakarta and not taken by the people who have no responsibility. The research is a normative and empirical legal research, the researcher elaborate the concept and principles of a library-based method. The data is collected from library. This library will be supported by the data taken from the field research by means of observation and an interview with the knowledgeable informant. The result of analysis data, there are two aspect,that is: The role of Special Region of Yogyakarta Government in Protecting of Traditional Knowledge and Mechanism to determine a traditional Knowledge