This study has porpouse to determine the effectiveness of molasses in the alternative media of cotton waste on the growth and productivity of white oyster mushrooms and determine the best concentration of molasses on the media of cotton waste against the growth of white oyster mushroom. This research uses RAL (Completely Random Design) with single factor experiment design consisting of 0,975 kg sawdust media treatment + 0,32 kg of brand, 1,3 kg cotton waste medium, 0,975 kg cotton waste medium + 0,32 kg of bran + 16.5 ml of molasses, 0,975 kg cotton waste media + 0,32 kg of bran + 33 ml of molasses, and 0,975 kg cotton waste media + 0,32 kg of bran + 50 ml of molasses. The parameters observed in this study were 100% mycelium growth time, mycelium development, baglog weight, contamination, first harvest time, number of fruit body, fresh weight of mushroom, harvest frequency, and total oyster mushroom yield. The results showed 0,975 kg cotton waste media + 0,32 of kg bran + 50 ml of molasses gave the best results on the total oyster mushroom yield of 459.93 gram, harvest frequency 6.13 times, and the number of fruit body that is 47.08.