Background: Adolescence is a time when people move from childhood to adulthood. At this time a person has a high curiosity and likes a challenge regardless of the consequences. Problems that often arise in adolescence, one of which is premarital sex behavior. The increasing number of pre-marital sexual behavior events lately is one of the things that troubles. One of the causal factors is lack of premarital sex prevention knowledge that is appropriate for teenagers so that adolescents perform premarital sex behavior. Research Purpose: This study aims to determine the prevention of premarital sex in health and non-healt student Research Method: Quantitative descriptive research design with cross sectional approach. Sampling technique using simple random sampling as many as 154 health students and 266 non-health students. This research use self-made questionnaires by researcher and have tested the validity of biserial correlation coefficient on 30 respondents with r table number 0,361 and reliability using KR-20 with result 0,786. This research was conducted at Muhammadiyah University of Yogyakarta. The results: Respondents of most of the late adolescents with age 20 years in healthcare students were 58 respondents (37.7%) whereas in non-health students mostly aged 19 and 20 years who had the same number as many as 87 respondents (32.7% . Most of the respondents came from Javanese area on health students as much as 100 respondents (64.9%) and on non-health students as many as 155 respondents (58.3%). Respondents who died in the health student in the kost 115 respondents (74.7%) and non-health as much as 209 respondents (78.6%). Respondents in the majority of health students have prevention of pre-marital sex with good category as many as 111 respondents (72.1%) whereas in the majority of non-health students have prevention of premarital sex with good enough category as much as 128 respondents (48.1%). Conclusions: Prevention of premarital sex on health students is better than non-health student.