Background: The patient safety culture at Pertamina Plaju Palembang Hospital still not good enough, it can be seen by the number of reporting patient safety incident from KPRS team. Whereas, this C-type hospital is obligated to implement patient safety culture in improving health service quality. Method: This research uses quantitative method with cross sectional research design. The number of research samples using slovin formula taken from medic and paramedic, medical support, and management and sampling with proportional stratified randong sampling. Measurement of patient safety culture using MaPSaF (Mancheste Patient Safety Framework) questionnaire which has been published NPSA (National Patient Safety Agency) in 206 and has been tested the validity and reability by previous research. The questionnaire consists of 10 dimensions with 24 aspect of the question. Result and Discussion: Implementation of patient safety culture at Pertamina Plaju Palembang Hospital in accordance with MaPSaF assessment of 70% at proactive level, 20% at generative level, and also 10% at bureaucratic level. Overall patient safety culture is dominant at the proactive level. Conclussions and Suggestions: The patient safety culture at Pertamina Plaju Palembang Hospital is the proactive level, but still needs improvement to the generative level with raising of awereness, good cooperation, and responsibility for the importance of patient safety culture. Keyword: patient safety culture, MaPSaF.