Background: Surgical safety checklist aims to decrease errors and adverse events, and to increase teamwork and communication in surgery. Surgical safety checklist is expected to decrease complication and death due to surgery. This study aimed to assess adherence to filling out surgical safety checklist among surgical related medical professionals at Nur Rohmah Hospital, Gunungkidul, Yogyakarta. Subjects and Method: This was a descriptive-quantitative conducted at Nur Rohmah Hospital, Gunungkidul, Yogyakarta. A sample of surgeons, anesthesiologists, surgery nurses, and anesthesia nurses, was selected for this study. The data related to adherence to filling out surgical safety checklist was measured by in-depth interview, checklist, observation, and document review. Results: Of the 25 surgeries performed (13 general surgery and 12 obsgyn surgery), 100% of the sign in phase surgical safety checklist was filled out, 83.42% of the time out phase surgical safety check list was filled out and 85.6% of the time out phase surgical safety check list was filled out. Conclusion: Adherence to phase surgical safety checklist was good at Nur Rohmah Hospital, Yogyakarta. However, there remains the need for supervision, development of standard operating procedure, socialization, and training, to improve adherence to surgical safety checklist for an improved patient safety. Keywords: surgical safety checklist, sign in, time out, sign out