The polymer materials of chitosan (CS) and polyvinyl alcohol (PVA) have attracted research interest in biomedical applications such as for wound dressing material due to their non-toxicity and biocompatibility. The electrospun nanofiber membranes made of a blended CS and PVA seemed to behave a relatively low tensile strength. In this research, therefore, the electrospun nanofiber membranes were prepared using the blended CS nano-emulsion (CSNe) and PVA to increase the tensile strength of the membrane. The effect of the addition of CSNe concentration into the PVA solution on the changes in nanofiber morphology has also been studied. Fabrication of the CSNe/PVA nanofiber membranes was began by preparing a PVA solution (10 wt. %) as a matrix. After that, the spinning solution of CSNe/PVA with various CSNe concentrations (0, 5, 10, 15 wt. 5%) was prepared by the addition of CSNe as fillers into the PVA solution. The electrospinning process of CSNe/PVA solution was carried out under the optimized conditions of applied voltage 15 kV, a fix distance from the syringe needle tip to a collector plate (TCD) 12 mm and internal diameter (ID) of the needle 8 mm. The viscosity and conductivity of the spinning solutions were measured using a viscometer and conductometer, respectively. Scanning electron microscope (SEM) was employed to characterize the morphology of the membranes and the membranes were tensile tested using a universal testing machine (UTM). The results indicated that the properties of spinning solutions affect the morphology of the nanofiber membranes. A decrease of the viscosity reduces the fiber diameter. Changes in the electrical conductivity of the spinning solution provide an impact on the uniformity of the fiber size ranging from 143.80 nm to 264.83 nm. The lowest (7.099 MPa) and highest (8.6404 MPa) tensile strengths were shown in the membrane with 0% and 15% CSNe concentrations, respectively. Whereas, the modulus elasticity of the membranes was ranging from 82.791 MPa to 108.365 MPa. The produced CSNe/PVA nanofiber membranes are expected to be useful as a wound dressing material shortly soon.