Background: Patient safety is an important but neglected issue in primary health care. The lack of research on safety culture in primary health care compared to hospitals and finding out how start-up clinic who has not been accredited, became the objective in this study. Methods: This research is a mixed methods research. The research quantitatively used patient safety culture questionnaire, adopted from AHRQ and qualitatively used deep interview. In order to conduct and analyse both of quantitative and qualitative methods, the researcher deployed sequential explanatory method. Results: From 12 dimension of patient safety, three dimensions in high score, 7 dimensions in medium score, but two dimensions were reporting system and punishment in low score. The major themes based on the deep interview conducted were the comprehension of patient safety culture of all staff and stakeholders, the implementation of patient safety programme, and management support. Conclusion: We concluded that accreditation is a sustaining factor that can be used as a framework for shaping patient safety culture. Patient safety is not a simple technique to gain quality in healthcare. We need continuous, multifaceted, and comprehensive approach to establish and maintain the implementation of safety culture. Keywords: Patien safety; Primary Health care