Background: Urinary catheter insertion is the most common cause of bacteriuria. The risk of bacteriuria in the catheter is estimated at 5% to 10% per day. UTI is the cause of >30% of all nosocomial infections. At least 80% of UTIs by invasive procedures or urinary tract equipment are usually catheterized. Knowledge level of each nurse is different, it is a factor causing of urinary catheter installationquality. Objective: This study purpose was to analyze installing urinary catheters knowledge before and after training. Methods: This research is an quasi experimental study that used a quantitative method (one group pretest-posttest design). The total population was 186 nurses and a sample of 52 people, with Simple Random Sampling technique. Univariate and bivariate data analysis using paired t test analysis. Results: The results showed that the knowledge of urinary catheter insertion prior to training was mostly in enough category of 36 respondents (69,2%). Nurses’ knowledge about urinary catheter placement during training was mostly in the good category with 42 respondents (80,8%). The Wilcoxon Test results were 0.000<0.05. Conclusion: There is a difference in urinary catheters insertion knowledge before and after training at PKU Muhammadiyah Bantul Hospital. Keyword : Training, urinary catheter insertion, nurse knowledge