Background: WHO has determined the importance of the application of precaution standards to health workers in every action to prevent an increase in Healthcare Associated Infections (HAIs). The use of personal protective equipment (PPE) is one of the important problems in the medical health service that so often being overlooked. Objective: The purpose of this research is to determine the effect of behavioral determinants on nurses' compliance with personal protective equipment at private hospital in Yogyakarta Methods: This study uses a mixed method method with Sequential Explanatory design. Quantitative research was carried out in advance by distributing questionnaires to assess behavioral determinants and observations to assess compliance with PPE use (using total sampling, n = 34). Then proceed with qualitative research using indepth interviews. Data analysis using multiple logistic regression analysis and constant comparative method. Result and Discussion: Statistical results show the factors that influence the use of PPE are knowledge p = 0.038; OR = 2.119, supervision p = 0.044, OR = 4.050 and motivation p = 0.046; OR 1.022. The dominant factor that most influences compliance with PPE is supervision. This is also supported by the results of qualitative research which explains that the use of PPE is related to support management where one of them is supervision. Conclusions: The use of PPE by nurses must continue to be improved. Management can develop various approaches to improve the health care workers behavior through increasing knowledge, motivation and supervision. Keywords: Behavior, Personal Protective Equipment, Compliance