This study aims to analyze the Empirical Study of Differences in Factors That Influence Interest Entrepreneurship in Management Students of Yogyakarta State University and Muhammadiyah University of Yogyakarta. The subjects in this study are the Management students of Yogyakarta State University and the Muhammadiyah University of Yogyakarta, class of 2014 who have taken entrepreneurship courses and have attended entrepreneurship seminars. In this study the number of respondents as many as 125 respondents selected by using the Slovin formula. Sampling method in this research is by using purposive sampling method that is determination of sample with certain criteria. This research uses Multiple Regression Analysis and Independent Samples T-Test which run through SPSS program as its analysis tool. Based on the analysis that has been done, the results obtained that: 1) Expectations Revenues have a positive and significant impact on Interest in Entrepreneurship, 2) Family Environment has a positive and significant impact on Interest in Entrepreneurship, 3) Entrepreneurship Education has a positive and significant impact on Interest in Entrepreneurship, 4) There is a significant difference of responses about expectation revenues in UNY Management students with UMY Management students, 5) There is a significant difference of responses about family environment in UNY Management students with UMY Management students, 6) There is a significant difference of responses about entrepreneurship education in UNY Management students with UMY Management students and 7) There is a significant difference of responses about interest entrepreneurship in UNY Management students with UMY Management students.