This study examines the Organizational Culture of the University of Muhammadiyah Yogyakarta in an effort to achieve the International Reputable University for the 2015-2018 period. The purpose of this study was to analyze the core values of UMY's organizational culture and understand how the application of organizational culture by all elements of UMY. Then examine how the process of internalizing UMY through the core values of organizational culture and its readiness to realize an international reputable university. The research method used is a qualitative method with a descriptive case study approach. This research is located at Muhammadiyah University of Yogyakarta. The source of research data comes from the results of interviews with informants, documents, and photo documentation. Test the validity of the data using source triangulation which means comparing and checking the level of trust in information obtained through time and different tools in qualitative research. The results of this study show that the nine core values of UMY's organizational culture include mandate and responsibility, togetherness, honesty, discipline, fairness, self-awareness, sincerity, caring, professionalism that has not been maximally implemented. This is due to the lack of socialization of activities that specialize directly in organizational culture. Then it does not have clear benchmarks related to the application of core values, the means of communication for the dissemination of organizational culture have not been effective. However, as a young campus, UMY has been considered ready to compete in the global world to become an international campus with the optimization of employess and core value of organizational culture.