Attitude toward reading in every person is different, especially for the students who are close with reading activity. There are three factors that can influence female and male students’ reading attitude. The factors are reading attitude by interest, reading attitude by preference, and reading attitude by frequency. This study aims to investigate the female students’ reading attitude, male students’ reading attitude, and also female and male students’ reading attitude differences in English Language Education Department of one of the universities in Yogyakarata. This is a quantitative study which involved 57 populations of female students and took the sample 49 female students and 34 populations of male students and took the sample 31 male students of English Language Education Department batch 2016 in one of the universities in Yogyakarta province. Questionnaire was used to get the data of female and male students’ reading attitude. The questionnaire was adapted from Artola, Sastre, Gratacós and Barraca (2013). Meanwhile, the mean the score of female students are 3,10 and the score of male students are 2,82. Both of female and male students’ reading attitudes were fair. The next finding was about the differences between female and male students’ reading attitude. The criteria for the significant different is if the value of Sig.( 2-tailed ) is < 0.05, then the value of Sig.( 2-tailed ) in this research is 0.005 which automatically shows that there are significant differences between female and male students’ reading attitude at English Language Education Department at Yogyakarta batch 2016. Thus, the alternate hypothesis is accepted because there is a significant difference between female and male students’ reading attitude at English Language Education Department batch 2016.