The purposes of this research are to multiply various sources of mycorrhizal inoculum and test its compatibility in three local varieties of Cassava in Gunungkidul and to examine the evolution of mycorrhiza on cassava seeds. mycorrhizal compatibility of various sources on three varieties of cassava. The experimental study was prepared in RAKL (Uncompletely Randomized Design) with a factorial design (3x3). Factor 1 is composed of 3 levels of mycorrhizaes from various sources as follows: A: Indigenous of Gunungkidul Mediteran soil; B: Rhizosphere of Bugel beach pandanus plants; C: commercial mycorrhizal inoculum. Factor 2 is cassava varieties consist of 3 levels, which are; P: Mentega; Q: Kirik; R: Ketan. The observed parameters were percentage of mycorrhiza infection on cassava root, number of spores on soil, root length, plant height, fresh weight of canopy, and number of leaves.The result indicated that the multiplication of mycorrhizal sources proven to infect the roots of cassava and there were the spores in various sources of mycorrhiza. This proves that there is an association between mycorrhizae and cassava root and the presence of planting media spores and based on the observation of spore identification. Spore types are Glomus sp., Gigaspora sp., And Acaulospora sp. Cassava varieties that have the best or effective response to mycorrhiza is Kirik varieties.