Background: In Indonesia,visual impairment occurs more frequentlyt in people of productive ages. Medical students are one group of people at risk of visual impairment. The autorefractometer is currently considered as a supportive instrument to estabilish the diagnosis of refractive disorders (myopia, hypermetropia, astigmatism) because of its superiority in time efficiency. Aim: This study was aimed to identify whether there is a diffrence between the examination of refractive abnormalities using autorefractometer comapared with the gold standard (trial lens). Methods: Cross-Sectional Study was conductied on 62 medical students of UMY to collect the data of refractive abnormalities using autorefractometer and trial lens. The normality test of the data was performed by using the Kolmogorov Smirnov Test. Data analysis was carried out by using the Mann-Whitney Test. Results: Based on the comparative results of Spherical Equivalent (SE), there was no significant difference between the examination of refractive with autorefractometer and with trial lens on 62 subjects.Tthe data analysis test using the Mann-Whitney Test showed p=0,06 for the right eye and p=0,11 for the left eye. Conclusion: There was no significant difference between the examination with autorefractometer and with trial lens on both eyes. A more negative result is obtained from refraction check using the autorefractometer.