Shift work is a system of working time which is given in turns within 24 hours, divided into 3 times that is morning, afternoon, and night. The high rate of work accident can occur because work time changes which is caused by fatigue, besides that it also has an impact on GDP level of personnel, which one the changes can cause health problems. The aim of the research is to determine the difference, the average value of fasting blood sugar levels of morning shift security, day shift, and night shift at Universitas Muhammadiyah Yogyakarta. The design of this research was descriptive comparative non-experimental. That is measuring the fasting blood sugar level with a cross sectional approach, with 21 respondents who were shift security workers in Universitas Muhammadiyah Yogyakarta. Data analysis techniques are carried out in univariate and bivariate. The statistical test was carried out by the Shapiro-Wilk normality test. And then to the Biravare analysis using the Independent-Samples Krukal-Wallis Test. The results obtained from the analysis that showed there were no difference in fasting blood sugar levels (0.425; p>0.05). Therefore the hypothesis is accepted.