Background: In Indonesia, more than 85% of road traffic accident victims die and 92% of them disable. Road traffic accidents in Yogyakarta increase tripled and each year it kills at least 130 people (12%). Road traffic injuries are divided into three: bruises /severe bruises (70.9%), sprains (27.5%), and fractures (23.2%). The lack of pre-hospital handling knowledge influences people’s trust to help road traffic accident victims. They will hesitate to help since they are worried to make mistakes when helping the victims. This makes them reluctant to do pre-hospital handling thus the number of disabled victims from road traffic accidents becomes large. Objective: The aim of the study was to identify the knowledge of pre-hospital fracture treatment using splint and bandage on traffic accident victims in Sleman. Method: The study was non-experimental quantitative research. The method of the study was a descriptive analysis. The study sampled 69 respondents with a purposive sampling technique. The data was analyzed using a univariate analysis. The instrument used two types of questionnaires to test the knowledge of split and bandage and the knowledge of pre-hospital fracture handling. Results: The results of the study showed that the level of knowledge about splint and bandage was good (81.2%) and the same went to the level of knowledge about pre-hospital fracture handling (79.7%). Conclusion: The respondents’ age mostly ranged from 17-25 years old or late adolescence. They were students or college students. The description of respondents’ knowledge level regarding pre-hospital fracture management was good. Fifty five respondents (79.7%) understood the knowledge. The description of respondents’ knowledge level regarding splint and bandage (the respondents who understood the knowledge of splint and bandage, the purpose of bandages and breeding, the principle of retribution and breeding, the types of bandages and splints and the complications of dressing and breeding) also showed the similar good result. Fifty six respondents comprehended the knowledge of splint and bandage (81.2%).