Settingintravenous cateter is the common procedure for inpatient in a hospital as intravenous therapy, drugs giving, fluid rehidration, and blood products. The intravenous equipments is induced local complication or systemic, liked trombophlebitis, endocarditis, and primary blood flows infection. The diseases are differntiated into infectious and non infectious , infection is invasive procces caused by microorganism and do poliferation inside the body that induced kinds of diseased. Non infectious chronic disease reported give a lot contribution is almost 60% globaly mortality number. The primary non infectious diseases are cardiovascular disease (30%), cancer (13%), chronic lungs channel disease (7%), and diabetic melitus (2%). This experiment is used analytic design by cross sectional method experimental based on 32 samples chosen at random by patients with diagnosis infectious diseased and non infectious who gets intravenous therapy in public regional hospital of Yogyakarta. The datas analytic that used by t-test to knowing the differentiation amount of germs number by intravenous canole the patients who diagnosis infectious diseased and non infectious. Based on analytic results by t-test experiment tool gets the p= 0,029 (p<0,05), means there was the significantly differentiation among the amount of germs number by intravenous canole patients who diagnosis infectious diseased and non infectious. There was the significanly differntiation by the germs number of intravenous canole patients who diagnosis infectious and the rates of germs by intravenous canole patients who diagnosis infectious is more than patients who diagnosis non infectious