Background: Dengue Hemorrhagic Fever (DHF) was one of the diseases caused by dengue virus transmitted by female mosquitoes of Aedes aegypti species (primary) and Aedes albopoictus (secondary) and every year there were always cases of DHF in Indonesia. The fluctuation of climates was likely to be related to the increasing number of cases with increasing trends and spreading in both urban and sub-urban areas. Hence it is important to know the relation between climates with dengue hemorrhagic fever in urban and sub-urban areas endemic in city and village. Method: These studies design was a non-experimental studies by using cross sectional approach. Datas taken was secondary data and for taking sample in this studies by using all data that written in Yogyakarta Health Office in sub-urban area was Seyegan and urban was Wirobrajan. The data was analyzed by using multipple linear regression test. Result: The results of the study found a significant value of climate influence with the incidence of DHF in urban areas are temperature p = 0.008 where p <0.01, rainfall p = 0.098 and humidity p = 0.082 where the value (p> 0.01). While for sub-urban areas are the temperature p = 0.764, rainfall p = 0.374 and humidity p = 0.463 where the value (p> 0.01). This means that in urban areas there is an influence of temperature on the incidence of DHF but there is no effect of rainfall and humidity on the incidence of DHF. Whereas in the sub-urban area there is no relations between temperature, rainfall and humidity to the incidence of DHF. Then obtained urban regression coefficient of 50,887+1,342 X1 – 0,005 X2 - 0,226 X3 and sub-urban area √ – . Conclusion: There is no significant relation between climates with dengue hemorraghic fever in urban and sub-urban areas in Wirobrajan and Seyegan