Polyvinyl alcohol (PVA) and chitosan are a combination of polymers commonly used as a wound dressing because they have biocompatible, biodegradable and non-toxic properties that can be developed to be the nanofiber membranes. Electrospinning is the easiest method and widely used in making nanofiber membranes. PVA/Chitosan membrane used as wound dressing must have native skin mechanical properties, i.e. high tensile strength (5-30 MPa) and medium modulus of elasticity (4.6-20 MPa). The purpose of this study was to determine the effect of adding chitosan nanoemulsion (NeCs) to on the morphology and mechanical properties of PVA/NeCs nanofiber membranes. Moreover, knowing the useful of PVA and PVA/NeCs membranes on wound healing in mice wounds. The electrospinning process was carried out at a voltage of 11 kV, the diameter of the syringe needle was 0.6 mm and the distance of the needle tip with the collector (TCD) was 12 cm. 10% PVA solution (% by weight) is used as a matrix mixed with NeCs using concentrations of 0%, 5%, 10% and 15% (weight%). The physical properties of the solution were tested using a conductometer and viscometer. Membrane morphology was observed using Scanning Electron Microscope (SEM), then examined its tensile properties using Universal Testing Machine (UTM). In vivo wound tests were carried out for 17 days using PVA / NeCs membranes compared to commercial wound dressing (Sofra-Tulle) and tested on mice wounds divided into 3 groups. Group I (pure PVA), group II (PVA / NeCs 15% / treatment) and group III (Sofra-Tulle). The results showed that the addition of NeCs decreases the viscosity, but it increases the conductivity of the PVA/NeCS solution. Tensile properties of PVA / NeCs membranes made using PVA low molecular weight approached native skin mechanical properties. The highest tensile strength was obtained from PVA/NeCs 5% membrane (13.68 MPa) and the lowest was found in PVA/NeCs 15% membrane (8.27 MPa). Modulus of elasticity increased from 36.69 MPa to 59.70 Mpa, whereas the effectiveness of wound healing test showed that 15% PVA/CsNe membrane was able to approach the commercial wound dressing capability