This study analyzes the representation of gender identities in the vlog of Jovi Adhiguna Hunter. This study aims to examine the representation of alternative gender identities presented by a vlogger named Jovi Adhiguna Hunter in a vlog uploaded via YouTube. The theory used as a theoretical framework in this study is gender identity, representation in social media and semiotics: reading signs in the media. The research method used in this study is the semiotic method with data analysis techniques namely semiotics John Fiske which refers to three levels, that is level of reality, level of representation and level of ideology. The results of this study indicate that Jovi Adhiguna Hunter tries to reconstruct the gender ideology that is understood by most people as limited to masculine and feminine. Gender identity that is represented is very fluid, not fixed, unstable and can change according to certain conditions and times. In addition, gender identity is not always the same as gender expression