Background: Estrogen deficiency is a condition that often occurs in society, especially in women who experience menopause. Microscopically the gingival epithelium in postmenopausal women experiences atrophy. Reduced estrogen hormone levels result in a lack of keratinization of the epithelium at the gingival margins and cause the gingival tissue to easily experience desquamation. The epithelium that is thinning on the gingiva will also cause the gingiva to become susceptible to irritation and inflammation. Ulcers are lesions formed by local damage from the epithelium tissue. Ulcers that form in the oral mucosa are a picture of oral lesions that are very common in most people of various ages and genders. Phytoestrogens are a group of non-steroidal compounds that can be derived from plants that have estrogen-like behavior. There are 4 different classes of phytoestrogens, namely isoflavones, lignans, coumestenes, and stilbenes. Isoflavones contained in legumes, especially in soybeans, all processed rice, potatoes, fruits, and vegetables, while lignans are a minor element in cell walls, the fiber in seeds, fruits, vegetables, rice, and beans. Method: This study used a laboratory experimental design in vivo using Sprague Dawley female mice as test animals. The number of samples in this study was 20 in 5 treatment groups consisting of 4 rats per group. The ELISA test was conducted to determine the concentration of estrogen in rat blood and the observation of the number of lymphocyte cells in the ulcer performed on days 1, 3, 5 and 7 with a microscope of 100x magnification with Hematoxylin-eosin (HE) staining on histological preparations. Results: The five groups carried out the Shapiro-Wilk normality test were obtained (p-value <0.05) which means that the data distribution was not normal and the Kruskal-Wallis test was obtained (p> 0.05) which means the average number of macrophage cells in all groups did not have a significant difference in the 7th day. The results of this study showed no significant difference between the numbers of cells of all groups on day 7. Conclusion: Giving soybean Tempe flour extract orally in Sprague Dawley ovariectomy rats for 30 days can increase the concentration of estrogen in the blood. Oral administration of soybean tempeh flour extract for 30 days on traumatic ulcer healing of female Sprague Dawley mice with estrogen deficiency affected the inflammatory phase which can be seen from the difference in lymphocyte counts, but the difference was not significant.