Tissue engineering technology, is one of the most advanced procedures in tissue healing methods which are able to initiate and maintain the regeneration process by involving cell regeneration, growth factors, and scaffold. The purpose of this study is to determine the effectiveness incorporation of Platelet Rich Plasma in scaffolds with gelatin-CaCO3 5:5, 4:6 and 10:0 membrane concentrations. The research Is a kind of experimental laboratories, with total samples of 9 scaffolds with 3 scaffold samples from each membrane concentration ratio. Each scaffold is 30μl of Platelet Rich Plasma. Calculation of the number of Platelet Rich Plasma using a binocular microscope. The results of Platelet Rich Plasma calculations were statistically by One-Way ANOVA test with the results of the difference in membrane concentration of 5: 5 (p = 0.001), concentration of 4: 6 (p = 0.000) and concentration of 10: 0 (p = 0.001). The results showed an average value of Platelet Rich Plasma in each scaffold with a concentration of 5:5 as many as 970.33, a concentration of 4:6 as many as 1,173.66 and a concentration of 10:0 as much as 740.33 so that there was a significant difference (p <0.05). The conclusion of this study is that there are differences in the number of Platelet Rich Plasma that has been incorcated in the scaffold with a comparison of the concentration of gelatin-CaCO3 5: 5, 4: 6 and 10: 0 membranes.