Cancer is one of the chronic diseases that cause death and is the leading cause of death in the world.Raji cells is from the Burkitt’s lymphoma cancer that infected by Epstein-Barr Virus (EBV) with very aggressive growth and development characteristics. Conventional cancer therapies such as chemotherapy, radiotherapy, surgery and combinations have a negative impact for the patient’s body. Searching for anticancer drugs as herbal therapies is expected to be effective and have minimal side effects. Ethyl acetate fraction from the content of active compounds from ant nest plants (Myrmecodia pendens Merr. & Perry), among others, such as flavonoids, saponins, and tannins have the potential as anticancer agents. This study aims to examine the potential cytotoxicity activity of ethyl acetate fraction of ant nest plants (Myrmecodia pendens Merr. & Perry) towards Burkitt's lymphoma cells. This type of research is pure laboratory research conducted in vitro. The research method by conducting cytotoxicity tests using the MTT Assay method with cell absorbance readings using ELISA (Enzyme Linked Immunosorbent Assay) microplate reader by setting a wavelength of 595 nm. Burkitts's lymphoma cells were treated with various concentrations of ethyl acetate fractions, namely 125; 250;500; and 1000 μg / ml. The results showed that there was not cytotoxic activity of ethyl acetate fraction of ant nest plants (Myrmecodia pendens Merr. & Perry) towards Burkitt's lymphoma cells because there was bias result that can’t prove there was cytotoxicity. The explanation descriptively the ethyl acetate fraction of ant nest plants (Myrmecodia pendens Merr. & Perry) towards Burkitt's lymphoma cells have the greatest cell death in lowest concentration (125 μg / ml) at 98.82 % that shows this data is bias because that results is not suitable with dose-dependent phenomenone. Based on the results of these studies, it can be concluded that the ethyl acetate fraction of the ant nest plant (Myrmecodia pendens Merr. & Perry) has not cytotoxic activity towards Burkitt's lymphoma cells in vitro.