Background:Red rose extract (Rosa damascene Mill) has substances such as tannin, geraniol, nerol, citronellol, flavonoid that can inhibit and destroy the bacteria, including Enterococcus faecalis. Enterococcus faecalis is able to survive in a high pH environment and survive in root canal that can invade dentinal tubules which cause Enterococcus faecalis to become pathogenic bacteria and cause failure of root canal treatment. The mechanism of bacterial inhibition is interferes with peptidoglycan transpeptidase activity so that the formation of cell walls is disrupted and cells undergo lysis. Objective:To find out the effect of antibacterial power of red rose extract (Rosa damascene Mill) on the growth of Enterococcus faecalis bacteria. Method: The design of the study was In Vitro experimental laboratory. The concentrations of red rose extract were 25%, 50%, 75%, and 100%. Calcium hydroxide as a positive control and aquades as a negative control. Antibacterial activity test was using diffusion disc during 24 hours in 37°C incubation. Statistical test was using the Kruskal Wallis test to find out the effect of antibacterial power of red rose extract (Rosa damascene Mill) on the growth of Enterococcus faecalis bacteria. Result: The avarage of radical zone formed in red rose extraxt (Rosa damascene Mill) with concentration of 25% was 4,048 mm, concentration of 50% was 5,165 mm, concentration of 75% was 6,185 mm, concentration of 100% was 8,895 mm, and positive control was 5,5 mm. Conclusion: This study concluded that the extract of red rose (Rosa damascene Mill) had an antibacterial effect on the growth of Enterococcus faecalis bacteria.