Background: Resistance becomes a global health problem with the impact of increasing morbidity, mortality, and health costs. The use of perioperative antibiotics with excessive tendency is one of the causes of resistance. It is necessary to evaluate the use of antibiotics in quantity and quality. The purpose of this study was to evaluate the use of antibiotics in perioperative patients who performed general surgical operations in the Teratai Room of dr. Sudirman Hospital Kebumen. Subjects and Methods: The study used a mixed method, with a sequential explanatory design, on the medical records of BPJS 3 patients with a sample of 280. The subjects in this study were all specialists in permanent General Surgery, Pharmacists, and hospital management. Data was collected retrospectively on medical records and interviews. Quantitative analysis using the DDD 100 patient days method and qualitatively using the Gyssens criteria. Results: The evaluation results of the quantity of antibiotic use in the hospital showed a total DDD / 100 patient-days of 26.49, the highest in ceftriaxone. The results of evaluating the quality of antibiotic use showed category 0 (right) 161 people (57.5%), the remaining 22 people (7.9%) category IVd (incorrect) and 97 people (34.6%) category V (without indication) . While the cost of using antibiotics Rp. 3774,776.88 with BPJS claims Rp.4,068,122.50. Conclusion: Doctors tend to prescribe antibiotics from the third generation cephalosporin (ceftriaxone). Not all doctors comply with the regulation of antibiotic use in hospitals. While the cost of treatment can be reduced if the quality of antibiotic use is improved.