Background: Efforts to inhibit cancer cells' growth in the oral cavity require effective strategies and potential. One of them uses the ant nest plant (Myrmecodia pendens Merr & Perry), a natural medicinal plant. It has been empirically and scientifically tested in its potential for antitumor. Herbal cancer treatment has advantages, i.e., minimal side effects than conventional therapies. Myrmecodia pendan Merr & Perry is a plant with the content of terpenoid, procyanidin B1, rosmarinic acid, alkaloid, flavonoid, and tocopherol compounds as anticancer compounds. Objective: To examine the anticancer activity of ant nest plants (Myrmecodia pendans Merr. & Perry) on the inhibition of signal transduction of the CDK-2-cyclin-E protein complex NF-kB in silico molecular docking’. Method: Using the main Auto DockVina application, in the silico molecular docking test. DS Visualizer was used to making and visualizing target proteins and test ligands. The molecular docking results measured root mean square atom position deviation (RMSD) values < 2.00Å. Results: The molecular docking test showed that in the ethyl acetate fraction compounds, terpenoid, procyanidin B1, rosmarinic acid, alkaloids, flavonoids, and tocopherols had docking scores with RMSD values < 2.00Å. Conclusion: Terpenoids, Procyanidin B1, rosmarinic acid, alkaloids, flavonoids, and tocopherols were active compounds in the M. Pendens fraction of ethyl acetate showing activity against its cancer. Ants Nest (Myrmecodia pendans Merr. & Perry) had anticancer activity in the protein complex CDD-2-cyclin-E and signal transduction barrier NF-kB: by the molecular docking test In Silico