Mental health disorders have become very serious problems, which happened in 450 million people in the world and more than 28 million people in Indonesia. According RISKESDAS (2013), Yogyakarta is one of province with the most problems with mental disorders. Communities in Indonesia still have negative perceptions about mental disorders as someone dangerous and the something that uncureable, it is due to lack of knowledge about mental disorders. Students as part of the public are generally divided into two, which are health students and non-health students. Health Students in the college gain knowledge about mental health, whereas non-health students get nothing. Objective: To know the perception of health students and non-health students toward people with mental disorders Methods: This research was a non-experimental quantitative with descriptive comparative cross-section design. Respondents consisted of 224 health students with simple random sampling technique and 224 non-health students by cluster sampling. The experiment was conducted in May to June 2016 at the Universitas Muhammadiyah Yogyakarta. Instrument used a questionnaire with Likert scale. Analysis hypotheses Non parametric test used Mann-Whitney. Results: Characteristics of the respondents in both groups were dominated by female, aged 17-25 years old and Javanese. Results of perception in both groups of students was dominated by good category as much as 178 respondents (79.5%) on health students and 200 respondents (89.3%) on non-health students. The results of the Mann-Whitney test analysis obtained p = 0.004 Conclusion: There are differences in perception between health student and non-health students toward people with mental disorders.