Anxiety is signal to someone which represents threat, that make someone cautious in choosing any movement. One of situation that can make someone, especially student, feeling anxiety is national exam. This anxiety feeling can appear when in preparation, and/or as the exam start, in impact someone can get difficultness when they are trying to solve national exam. Statistics from Ministry of Education show that 2.335 didn’t pass the junior high school national exam in 2014. Aims : To analyze the effect of listening Al-Quran or murottal along with read the translation in bahasa in level of anxiouxity in 3rd grade junior high school students of Mu’allimaat Muhammadiyah Yogyakarta when they are prepared before national exam. Methods : Experimental method in pre-test and post-test called TMAS (Taylor Manifest Anxiety Scale). This research implemented in 65 junior high school students MTs Mu’allimat Muhamadiyah Yogyakarta whose separate in two groups, 33 students for intervention group and 32 students for control group. In intervention group, students listened Al-Quran surah Ar-Rahman along with read meaning of verses for 14 days, while in control group not be given any intervention Result : There is significant decreasing in average level of anxiety in pre-test and post-test intervention group with scale 0,028 (>0,05). While in control group it has significant increasing with value 0,000 (<0,05). Conclusion : Listening to Al-Quran along with read meaning of verses has positive effect decreasing level of anxiety for junior high school students when facing national exam.