This research aims to analyze the influence of role conflict on performance of employees that is mediated by work stress study on the Financial management of the Agency's employees and assets of the area.This research uses a quantitative approach with the method of dissemination of the now. The subjects in this study were in the offices of Agency financial management and assets Area of yogyakarta.The number of samples that are used as much as 102 respondents using census methods.Analytical techniques used for this test is the Structural Equation Modeling (SEM) using software AMOS version 22. Based on the analysis that has been done then obtained the results that the influential role conflict negatively to the performance of the employee, the influential role of positive conflict against work stress, work stress the negative effect on performance of employees, as well as influential role conflict negatively to the performance of employees through the mediation of work stress on employees of the Agency's financial management and assets Area of Yogyakarta.