This study aims to analyze the effect of leader-member exchange, work-life balance, and job satisfaction on organizational citizenship behavior. Respondents in this study were nursing staff at Muntilan District Hospital Magelang Regency, Central Java. The sample used was 169 respondents and was taken using purposive sampling technique. Data analysis techniques used the SEM (Structural Equation Modeling) method with the application AMOS 22.0. In this study, the Sobel test was also conducted to determine the significance level of mediation role. Based on the results of data analysis, this study found that leader-member exchange has a positive and significant effect on job satisfaction, work-life balance has a positive and significant effect on job satisfaction, job satisfaction has a positive and significant effect on organizational citizenship behavior, leadermember exchange has a positive effect and significant to organizational citizenship behavior, work-life balance has a positive and significant effect on organizational citizenship behavior, job satisfaction mediates the influence of leader-member exchange on organizational citizenship behavior significantly, and job satisfaction mediates the effect of work-life balance on organizational citizenship behavior significantly .