The Yogyakarta-Wonosari road segment is one of the primary arterial roads that has high mobility, then causing frequent accidents to the road. This is the main reason for a road safety audit to identify and analyze the situations which are very risky or have the potential to cause accidents. Hence, the research aim is; to identify accident-causing characteristics, to evaluate and assess potential problems in road safety operations, to analyze JPH and JPM 4, and to be able to provide recommendations for the further improvements to the Yogyakarta-Wonosari road. By collecting and analyzing primary and secondary data, the highest number of accident victim was 155 people and the most frequent cause was because of human, at 91 cases. Moreover, based on the age of the victims, the highest number of accidents is on the age of 26-35 years old, with 87 people, while based on sex, men are likely to be a victims, for 201 people. On the other hand, the types of vehicles that were most involved in accidents were motorbikes, 164, and based on the case process, the most common types of incidents were the two-vehicle accidents without turning movements, for 57 events. Thus, it can also be concluded that the visibility, 41.81m and 43.96m, is not safe due to its value which is smaller than the planed visibility that is equal to 84.64 m, while overtaking visibility is also not safe because the value, 193.51m and 201.96m, is smaller than the overtaking visibility on the plan, which is 302.68m.