A film is one of the media that used as a medium of da'wah by the director in delivering messages to the audience. Through a film, a director can incorporate religious values to be imitated by the audience through dialogues between characters in the scene. As with Guntur Soeharjanto with his bestseller film work, Ayat-Ayat Cinta 2. Therefore, this research aims to analyze Islamophobia in the film to know how to counter Islamophobia itself. The benefits of this study directed to the public so they can pay attention and choose the right way to convey messages to others. Furthermore, this research is expected to provide recommendations on the use of media to support good Islamic broadcasting. This study uses a qualitative approach. The analysis used is Roland Barthes's semiotic analysis with its signified and signifier and the connotation and denotation meanings