AIDS caused by HIV is a health issue of global concern. In Indonesia, in the first quarter of 2017, the number of HIV cases was 10,376 and AIDS cases were 673. DIY is a tourism city and student city that has a high level of human movement so it is highly likely that behaviors that are at risk of contracting or transmitting HIV and AIDS. Therefore, collaborative governance among stakeholders is needed in HIV and AIDS prevention. There are eight indicators to see the success of collaborative governance according to DeSeve: Networked Structure, Commitment to a Common Purpose, Trust Among the Participants, Governance, Access to Authority, Distributive Accountability / Responsibility, Information Sharing, Access to Resources. The findings of this study are that cross-sectoral collaboration carried out does not yet have regulations as a guideline in carrying out such collaboration. So that cooperation is only at the stage of mutual involvement in HIV and AIDS prevention, not at the stage of official cooperation that has legality. Because the existence of basic rules is important in collaborative governance as the initial foundation and legitimacy for stakeholders who act. The ease of accessing information, transparency and information sharing among the stakeholders involved in this collaboration will affect the implementation of cross-sectoral programs and activities. By sharing information, easy access to information, coordination will run smoothly, and transparent cooperation shows that the implementation of HIV and AIDS prevention can be accounted for and carried out accordingly