This study aims to analyze the role of job satisfaction in mediating the influence of work to family conflict and family to work conflict on the performance of nurses on female nurses in families of PKU Muhammadiyah Gamping. Subjects in this study were female nurses who have married. Object in this research PKU Muhammadiyah Gamping Hospital. In this study a sample of 72 respondents taken using the method of saturated sampling or commonly called the census. Analyzer used is Partial Least Square (PLS). Based on the analysis that has been done, the result shows that work to family conflict have negative and significant effect on job satisfaction, family to work conflict have positive and insignificant effect on job satisfaction, work to family conflict have positive and insignificant effect on performance, family to work conflict have influence negative and significant to job satisfaction, job satisfaction has positive and significant effect on performance, job satisfaction does not mediate relationship between work to family conflict on performance, job satisfaction does not mediate relationship between family to work conflict to performance.