Background: Health-care Associated Infections (HAIs) or Nosocomial Infection is infections obtained by patients or hospital workers. Some factors that cause HAIs are high-risk actions such as surgery, hemodialysis, lack of universal precaution like using Personal Protective Equipment (PPE). Aim: to know the effectiveness of simulation use of PPE to knowledge and compliance of use PPE in nurses in hemodialysis units. Method: The type of research used was mixed methods. Population consisted of all nurse in hemodialysis units and used totally sampling. Result: Nurses’ knowledge before simulation majority was categorized as sufficient (66,6%) and after the simulation, it was categorized as good (100%). Nurses’ compliance before the simulation was categorized as non-compliant (100%) and after the simulation, it was categorized as compliant (66,6%). The effectiveness of the simulation was analyzed using Wilcoxon test, obtained the results for the knowledge of pre-post simulation value of Sig.0,046 and the compliance of pre-post simulation value of Sig. 0,046. The obstacles in the use of PPE include the lack of special PPE training, monitoring and evaluation that have not been carried out properly, and the availability of PPE that is not sufficient. Conclusion: The simulation of PPE use is effective in increasing knowledge and compliance of the PPE use. Keyword: Personal Protective Equipment (PPE), Knowledge, Compliance